Streamlined duplex live-dead microplate assay for cultured cells

Microplate assay for measurement of histamine release from mast cells.

A microplate-format assay for measurement of histamine release by activated rat peritoneal mast cells, with accompanying microplate-format spectrofluorometric assay of histamine, is demonstrated. Reproducibility and linearity of the histamine assay are comparable to the historical large format assay. Transfer of samples in the 96-well format, from histamine release through spectrofluorometric a...

Dendritic Cells Pulsed with Live and Dead

A rapid and specific microplate assay for the determination of intra- and extracellular ascorbate in cultured cells.

Vitamin C (ascorbate) plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. For instance, within the brain, ascorbate acts in a neuroprotective and neuromodulatory manner that involves ascorbate cycling between neurons and vicinal astrocytes--a relationship that appears to be crucial for brain ascorbate homeostasis. Additionally, emerging ...

Microplate alamarBlue assay for Paracoccidioides susceptibility testing.

CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and ca...

Live and Dead Nodes

In this paper, we explore the consequences of a distinction between ‘live’ and ‘dead’ network nodes; ‘live’ nodes are able to acquire new links whereas ‘dead’ nodes are static. We develop an analytically soluble growing network model incorporating this distinction and show that it can provide a quantitative description of the empirical network composed of citations and references (inand out-lin...